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17 Alpha-hydroxy Progesterone

Endocrine & Reproductive

17-hydroxyprogesterone17-OHP17-OH Progesterone

Review status

Currently under review

Pending specialist review and validation.

What it shows

17 Alpha-hydroxy progesterone (17-OHP) is a steroid hormone made mainly by the adrenal glands, with smaller amounts from the ovaries or testes. It is a building block in the pathways that produce cortisol and androgens. Because it sits in the middle of these pathways, changes in enzyme activity can cause 17-OHP to rise or fall.

This blood test measures the amount of 17-OHP circulating in your body. It is commonly used to evaluate adrenal function, to screen or investigate congenital adrenal hyperplasia, and to help explain symptoms related to androgen excess. It may also be used to monitor treatment in people with known adrenal disorders.

Why it matters

Doctors use 17-OHP testing to look for conditions where cortisol production is impaired and steroid precursors build up, such as congenital adrenal hyperplasia due to 21-hydroxylase deficiency. In newborns, timely testing helps identify serious forms that can affect salt balance and overall health. In children and adults, it can help explain early pubertal changes, acne, or excess hair growth.

In women with irregular periods, infertility, or signs of androgen excess, 17-OHP can help distinguish among possible causes. The test also guides treatment in those already diagnosed, helping to balance therapy and avoid under- or overtreatment. Your clinician will interpret the result in the context of your age, symptoms, and other labs.

Understanding your results

Results are interpreted alongside your age, time of day the sample was drawn, menstrual cycle phase if applicable, and overall health. Laboratories use different methods, so comparing results over time is most reliable when testing is done the same way and at similar times of day. Your clinician may recommend repeating the test or using a specialized stimulation test to clarify borderline or unexpected results.

A higher level may prompt additional evaluation for adrenal enzyme deficiencies, consideration of genetic testing, or assessment for other adrenal or ovarian conditions. A lower level during treatment may indicate adequate control, although levels that are too low can suggest overtreatment. If your result does not match your symptoms, your clinician may adjust timing, review medications, or order complementary hormone tests before deciding on next steps.

Reference ranges

049.9 nmol/L
All sexes
0 days – 2 months
5.729 nmol/L
All sexes
2 months – 4 months
0.312 nmol/L
All sexes
4 months – 1 year
27 nmol/L
All sexes
1 year – 3 years
-- nmol/L
All sexes
3 years – 150 years

Reference intervals vary by laboratory, analyzer, methodology, population, and units. The ranges shown here are for education only. Always interpret your results against the reference interval printed on your own lab report.

Factors that could impact 17 Alpha-hydroxy Progesterone

  • Timing and circadian rhythm

    17-OHP follows a daily pattern with higher levels in the morning. Drawing blood early in the day and using consistent timing improves interpretation.

  • Menstrual cycle phase

    In people who menstruate, luteal phase levels can be higher. Many clinicians prefer sampling in the early follicular phase for consistency.

  • Illness and stress

    Acute illness, significant stress, or recent hospitalization can alter adrenal hormone production and transiently affect 17-OHP results.

  • Medications

    Glucocorticoids lower 17-OHP, while adrenal stimulation (for example, ACTH tests) can raise it. Oral contraceptives and antiandrogens may also influence results.

  • Prematurity and newborn factors

    Premature or ill newborns can have higher baseline values. Repeat testing after stabilization is often needed to avoid false positives.

  • Pregnancy

    Physiologic changes in pregnancy can increase steroid precursors. If you are pregnant, your clinician will interpret values within that context.

  • Assay and laboratory variation

    Different methods and reference materials can give slightly different values. Using the same lab and method supports consistent follow-up.

  • Liver or kidney disease

    Chronic liver or kidney conditions can modify hormone metabolism and binding, which may influence measured 17-OHP levels.

2026

References

  1. McGill University Health Centre. (2016, January 20). 17 Alpha-hydroxy Progesterone (Task CD 692258). Laboratory reference ranges.
  2. Speiser, P. W., Arlt, W., Auchus, R. J., Baskin, L. S., Conway, G. S., Merke, D. P., Meyer-Bahlburg, H. F. L., Miller, W. L., Murad, M. H., Oberfield, S. E., White, P. C., & Endocrine Society. (2018). Congenital adrenal hyperplasia due to steroid 21-hydroxylase deficiency: An Endocrine Society clinical practice guideline. The Journal of Clinical Endocrinology & Metabolism, 103(11), 4043–4088.
  3. Mayo Clinic Laboratories. (2023). 17-Hydroxyprogesterone, serum: Test ID OHPG. Laboratory test catalog.