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Beta 1 Fraction

Proteins & Electrophoresis

Beta-1Beta-1 globulinTransferrin fraction

Review status

Currently under review

Pending specialist review and validation.

What it shows

The Beta 1 Fraction is part of a serum protein electrophoresis test, which separates the proteins in your blood into groups based on their movement in an electric field. The beta-1 fraction is made predominantly of transferrin, the protein that carries iron in the bloodstream, with minor contributions from other beta-region proteins.

This measurement helps describe the overall protein pattern in your blood, alongside albumin, alpha fractions, beta-2, and the gamma region. It is not a stand-alone test and is interpreted together with the full electrophoresis tracing and your medical history.

Why it matters

Clinicians look at the beta-1 fraction when evaluating anemia, suspected iron imbalance, chronic inflammation, liver or kidney disease, malnutrition, or when an electrophoresis pattern is abnormal. Changes in this fraction typically reflect how much transferrin your body is making or losing and may point to conditions that require further evaluation.

In the assessment of monoclonal protein disorders, the full pattern across the beta and gamma regions is most informative. The beta-1 fraction by itself more often reflects nutritional status, iron status, or liver function rather than a monoclonal protein. Reviewing this value along with iron studies, a liver panel, and other tests helps clarify the cause of an abnormal result.

Understanding your results

A higher beta-1 fraction often tracks with increased transferrin, which can occur when your body is trying to transport more iron or when estrogen levels are higher from pregnancy or certain medicines. A lower value can be seen with poor protein intake, protein loss through the kidneys or gut, or reduced liver production. Because transferrin is a negative acute phase reactant, active inflammation can also lower this fraction.

Your clinician will interpret the result in the context of the entire electrophoresis tracing and your symptoms. If the finding is unexpected, follow-up tests may include iron studies, a complete blood count, liver and kidney tests, inflammatory markers, or, if an abnormal band is suspected elsewhere in the tracing, immunofixation. Most isolated changes in this fraction are not emergencies, but they provide useful clues to guide next steps.

Reference ranges

3.45.3 g/L
All sexes
0 days – 150 years

Reference intervals vary by laboratory, analyzer, methodology, population, and units. The ranges shown here are for education only. Always interpret your results against the reference interval printed on your own lab report.

Factors that could impact Beta 1 Fraction

  • Fasting and sample quality

    Nonfasting is generally acceptable, but marked lipemia or hemolysis can distort the electrophoresis pattern; proper collection and prompt serum separation help prevent artifacts.

  • Iron status and inflammation

    Transferrin rises when iron availability is low and typically falls during inflammation as a negative acute phase reactant, which directly influences the beta-1 fraction.

  • Hormones and medications

    Estrogen-containing therapies, pregnancy, and some anabolic or androgen treatments can shift liver protein synthesis, changing transferrin and the beta-1 fraction.

  • Liver and kidney function

    Reduced liver synthesis or protein loss from kidney disease can lower beta-1 levels; chronic liver disease and nephrotic states are common contributors.

  • Timing of therapy

    Recent iron supplementation or blood transfusion can alter iron balance and protein composition, which may change the beta-1 pattern during clinical follow-up.

  • Specimen type and handling

    Serum is preferred for electrophoresis; using plasma or samples with fibrinogen present, or delays in processing, can subtly alter separation and apparent beta fractions.

2026

References

  1. McGill University Health Centre. (2006, April 07). Beta 1 Fraction (Task CD 743401). Laboratory reference ranges.
  2. Rajkumar, S. V., Dimopoulos, M. A., Palumbo, A., Blade, J., Merlini, G., Mateos, M. V., Kumar, S., Hillengass, J., Kastritis, E., Richardson, P., Landgren, O., Paiva, B., Dispenzieri, A., Weiss, B., LeLeu, X., Zweegman, S., Lonial, S., Rosinol, L., Zamagni, E., ... & International Myeloma Working Group. (2014). International Myeloma Working Group updated criteria for the diagnosis of multiple myeloma. The Lancet Oncology, 15(12), e538–e548.
  3. American Association for Clinical Chemistry. (2023). Serum protein electrophoresis. Lab Tests Online.