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CSF Serine

Body Fluids

Cerebrospinal fluid serineCSF amino acid: serineCSF SerSerine, CSF

Review status

Currently under review

Pending specialist review and validation.

What it shows

CSF Serine measures the amount of the amino acid serine in your cerebrospinal fluid. Serine helps build cell membranes, supports myelin production, and participates in pathways that make neurotransmitters used for brain signaling.

Because cerebrospinal fluid surrounds the brain and spinal cord, this test reflects serine availability within the nervous system, which can differ from what is seen in blood. It is often ordered together with plasma and sometimes urine amino acid profiles to compare patterns across body compartments.

Why it matters

Clinicians use this test when there are signs that suggest a neurometabolic condition, such as seizures, developmental delay, or concerns about abnormal brain development. In particular, it helps evaluate suspected disorders of serine biosynthesis, in which the brain may not make enough serine. Recognizing this pattern early can guide treatment and support timely genetic testing and counseling.

The test can also help monitor the effect of nutritional therapy, such as serine or glycine supplementation, and assess whether treatment is reaching the nervous system. Results may guide additional testing, including gene panels, and help distinguish conditions that primarily affect the central nervous system from those that are systemic.

Understanding your results

Your report will show your result alongside an age-specific reference range from the testing laboratory. Interpreting the result involves looking at your symptoms, examination findings, and related tests, including plasma amino acids and other cerebrospinal fluid studies.

A result lower than expected can raise concern for a serine biosynthesis disorder, particularly when the cerebrospinal fluid value is more affected than blood. Your clinician may discuss starting supplementation, repeating the test to confirm, or pursuing genetic testing. A higher than expected result is less common and can occur with recent supplementation or if the spinal fluid sample was contaminated with blood during collection.

If a result does not seem to fit your clinical picture, try not to worry. Collection issues, medications, and timing can influence measurements. Your care team may repeat the test or add companion studies before making treatment decisions.

Reference ranges

3274 umol/L
All sexes
0 days – 6 months
2555 umol/L
All sexes
6 months – 6 years
2044 umol/L
All sexes
6 years – 150 years

Reference intervals vary by laboratory, analyzer, methodology, population, and units. The ranges shown here are for education only. Always interpret your results against the reference interval printed on your own lab report.

Factors that could impact CSF Serine

  • Blood contamination at lumbar puncture

    Red blood cells introduced during a difficult spinal tap can leak amino acids from blood into the cerebrospinal fluid, falsely increasing serine. Laboratories may check cell counts or other markers to assess contamination.

  • Age and developmental stage

    Normal cerebrospinal fluid serine varies with age, especially in infants and young children. Results are interpreted using age-specific reference intervals from the testing laboratory.

  • Diet and supplementation

    Recent intake of serine or glycine supplements, medical foods, or high-protein formulas can influence measured levels. Your clinician may ask about products you take to interpret results correctly.

  • Medications and parenteral nutrition

    Certain therapies, including total parenteral nutrition or some antiepileptic regimens, can alter amino acid balance. Provide a full medication and nutrition history before testing.

  • Inflammation or barrier changes

    Infections, inflammation, or conditions that affect the blood–brain barrier can change cerebrospinal fluid composition and may shift amino acid levels independent of a metabolic disorder.

  • Sample handling and storage

    Delays in processing, incorrect tube type, or inadequate freezing can affect measured amino acid concentrations. Proper collection and prompt freezing help ensure reliable results.

2026

References

  1. McGill University Health Centre. (2015, February 04). CSF Serine (Task CD 693391). Laboratory reference ranges.
  2. van der Crabben, S. N., Verhoeven-Duif, N. M., & de Koning, T. J. (2013). Serine Deficiency Disorders. In M. P. Adam et al. (Eds.), GeneReviews. University of Washington, Seattle.
  3. American College of Medical Genetics and Genomics. (2018). Laboratory standards for biochemical genetic testing and reporting of inherited metabolic diseases: A practice resource. Genetics in Medicine.