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Factor V Activity (1:40)

Coagulation

Coagulation factor V assayF5 activityFactor V activityFV Activity

Review status

Currently under review

Pending specialist review and validation.

What it shows

The Factor V activity test measures how well factor V, a clotting protein made in the liver, functions in your blood. It uses a clot-based method that compares your plasma to a normal reference, and reports the result in units per milliliter.

The notation 1:40 refers to a standard dilution of your plasma used by the laboratory to optimize accuracy and comparability. This test is commonly performed when initial clotting screens such as the PT or aPTT are abnormal, or when there are signs or symptoms of a bleeding tendency.

Why it matters

Too little factor V activity can be linked to easy bruising, nosebleeds, heavy menstrual bleeding, bleeding after procedures, or less commonly deep tissue bleeds. Low activity may be inherited or acquired due to conditions such as significant liver disease, disseminated intravascular coagulation, certain autoimmune inhibitors, or dilution after major transfusion.

Your clinician may order this test to investigate prolonged screening tests, a personal or family history of bleeding, or to assess for an inhibitor against factor V. Higher than expected results are uncommon and usually not worrisome on their own; they can reflect acute inflammation or technical issues rather than a true tendency toward clotting.

Understanding your results

If your activity is lower than expected, your clinician may repeat the test, review medications, and consider a mixing study to see whether adding normal plasma corrects the clotting time. Correction points toward a deficiency, while lack of correction suggests an inhibitor. Additional evaluations, such as other factor levels and liver function tests, may help determine the cause and guide treatment.

If your activity is within the expected range, it supports normal factor V function. If it is higher than expected, your clinician will interpret it in context, considering acute illness or assay interference. Results are best understood together with your symptoms, other coagulation tests, and medical history. Do not start or stop medications without medical advice.

Reference ranges

0.51.5 U/mL
All sexes
0 days – 150 years

Reference intervals vary by laboratory, analyzer, methodology, population, and units. The ranges shown here are for education only. Always interpret your results against the reference interval printed on your own lab report.

Factors that could impact Factor V Activity (1:40)

  • Sample collection and handling

    Underfilled citrate tubes, difficult draws, heparin contamination from lines, or delays in processing can falsely alter activity results. Proper fill, prompt centrifugation, and avoiding line draws when possible help ensure accuracy.

  • Anticoagulant medications

    Heparin and some direct oral anticoagulants can interfere with clot-based assays and make factor activity appear lower than it truly is. Warfarin typically does not lower factor V because this factor is not vitamin K dependent.

  • Liver disease and consumption

    Because factor V is made in the liver, significant liver dysfunction can reduce activity. Conditions that consume clotting factors, such as disseminated intravascular coagulation, can also lower measured levels.

  • Inhibitors and antibodies

    Acquired inhibitors to factor V may develop after exposure to certain surgical materials, infections, or medications. These antibodies interfere with function and are often detected by a mixing study that fails to correct.

  • Transfusion effects

    Recent transfusion of plasma or large volumes of intravenous fluids can temporarily change measured activity by replacing or diluting your own clotting factors. Timing of the blood draw relative to transfusion matters.

  • Age, pregnancy, and illness

    Physiologic changes in newborns, pregnancy, or acute illness can influence clotting tests and the way the assay behaves. Your clinician will interpret results in the context of these situations and your overall health.

2026

References

  1. McGill University Health Centre. (2015, March 01). Factor V 1:40 (Task CD 701482). Laboratory reference ranges.
  2. Clinical and Laboratory Standards Institute. (2008). Collection, transport, and processing of blood specimens for testing plasma-based coagulation assays and molecular hemostasis assays (5th ed., CLSI document H21-A5).
  3. Kitchen, S., McCraw, A., & Echenagucia, M. (2010). Diagnosis of hemophilia and other bleeding disorders: A laboratory manual (2nd ed.). World Federation of Hemophilia.