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Fetal Cells

Immunology & Autoimmune

Fetomaternal hemorrhage testKBKB testKleihauer-Betke test

Review status

Currently under review

Pending specialist review and validation.

What it shows

The fetal cells test measures the proportion of fetal red blood cells circulating in a maternal blood sample. It is commonly performed using the Kleihauer-Betke acid elution method, which highlights cells containing fetal hemoglobin on a microscope slide for counting.

Clinicians use this test around delivery, after abdominal trauma, or following invasive procedures in pregnancy to assess fetomaternal hemorrhage.

Why it matters

Detecting fetal cells that have entered your bloodstream helps your care team estimate the degree of fetomaternal bleeding. For people who are Rh negative, this information guides the dose of Rh immune globulin to lower the risk of Rh sensitization in the current and future pregnancies.

The test can also inform management after trauma, placental complications, or procedures, and may prompt additional monitoring for you and your baby.

Understanding your results

Your report will indicate whether fetal cells are present and provide an estimate of their proportion. A trace amount can be seen even without complications, while higher results suggest more significant fetal blood transfer.

Your clinician will interpret the result together with the timing of the sample, your Rh type, delivery or injury details, and any procedures. If needed, you may receive Rh immune globulin, have repeat testing, or undergo additional evaluation, but most results are addressed with routine care.

Reference ranges

00.03 %
All sexes
0 days – 150 years

Reference intervals vary by laboratory, analyzer, methodology, population, and units. The ranges shown here are for education only. Always interpret your results against the reference interval printed on your own lab report.

Factors that could impact Fetal Cells

  • Timing of the sample

    Collecting blood too soon after a bleeding event may miss redistribution, and collecting much later can underestimate results as fetal cells clear from circulation.

  • Maternal hemoglobin variants

    Conditions that increase fetal hemoglobin in adults, such as hereditary persistence of fetal hemoglobin or some thalassemias, can cause falsely high results with the Kleihauer-Betke method.

  • Recent transfusion or significant maternal bleeding

    Transfusion can dilute or complicate interpretation, and large maternal blood loss may alter calculated estimates of fetal cell proportion.

  • Multiple gestation or placental complications

    Twins or placental problems can increase the chance of fetomaternal bleeding, and results may lead to adjusted management or dosing decisions.

  • Laboratory method differences

    Manual Kleihauer-Betke staining and flow cytometry have different sensitivities and potential inter-observer variability, which can affect reported values.

  • Rh immune globulin and related testing

    Prior Rh immune globulin can influence some screening assays used with this test algorithm, so telling your care team about recent doses helps accurate interpretation.

2026

References

  1. McGill University Health Centre. (2012, January 12). Fetal Cells (Task CD 3845134). Laboratory reference ranges.
  2. American College of Obstetricians and Gynecologists. (2017). Prevention of Rh D alloimmunization (ACOG Practice Bulletin No. 181).
  3. British Committee for Standards in Haematology. (2009). Guidelines for the estimation of fetomaternal haemorrhage.