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Homocysteine Total

Metabolic Disorders

Plasma homocysteineSerum homocysteinetHcyTotal homocysteine

Review status

Currently under review

Pending specialist review and validation.

What it shows

Homocysteine is an amino acid produced when your body breaks down methionine. The total homocysteine test measures the combined amount circulating in your blood, including free and protein-bound forms. Your body uses folate, vitamin B12, and vitamin B6 to process homocysteine, keeping levels in balance.

This test is done on a blood sample from a vein. It is often ordered with tests for folate and vitamin B12, or as part of an evaluation for certain inherited metabolic conditions, cardiovascular risk assessment in select situations, or unexplained neurological symptoms.

Why it matters

Higher homocysteine can be a sign of low folate, vitamin B12, or vitamin B6, reduced kidney function, low thyroid function, certain medications, or rare genetic variants that affect homocysteine metabolism. Identifying the cause helps guide treatment, such as addressing nutrient gaps or other medical issues.

In some people, elevated homocysteine is associated with a higher likelihood of heart and blood vessel problems or blood clots. Routine screening for everyone is not recommended, but your clinician may order this test if there is concern about vitamin status, unexplained anemia or neuropathy, a known or suspected inherited homocystinuria, or selected risk assessments.

Understanding your results

Your result is considered together with your symptoms, medical history, kidney and thyroid tests, and vitamin markers. If your value is higher than expected, your clinician may check folate and vitamin B12, review your medicines, and consider kidney function or genetic causes.

If a reversible cause is found, treating it often lowers homocysteine. You may be asked to repeat the test, ideally after an overnight fast and with prompt sample handling, to confirm a persistent change. Do not start or stop vitamins or medications without discussing them with your clinician.

If homocysteine is substantially increased or if there are signs of an inherited condition, you may be referred to a metabolic or genetics specialist. If your result is within the expected range, no specific action is usually needed beyond maintaining a balanced diet and regular follow up as advised.

Reference ranges

4.615.5 umol/L
All sexes
0 days – 150 years

Reference intervals vary by laboratory, analyzer, methodology, population, and units. The ranges shown here are for education only. Always interpret your results against the reference interval printed on your own lab report.

Factors that could impact Homocysteine Total

  • Fasting and sample timing

    Recent meals, especially protein, can transiently raise homocysteine. Fasting before the blood draw is often recommended to improve consistency.

  • Sample handling and temperature

    Homocysteine can rise in the tube if plasma is not separated quickly or kept cool. Prompt processing reduces the chance of a falsely high result.

  • Vitamin status

    Low folate, vitamin B12, or vitamin B6 can elevate homocysteine. Addressing deficiencies with diet or supplements may lower the level.

  • Kidney function

    Reduced kidney function slows clearance and is a common nonnutritional cause of higher homocysteine results.

  • Thyroid status

    Untreated hypothyroidism can increase homocysteine. Correcting thyroid function may bring the value closer to expected levels.

  • Medications and exposures

    Methotrexate, some antiepileptics, nitrous oxide exposure, and high-dose niacin can raise homocysteine. B-vitamin supplements usually reduce it.

  • Lifestyle factors

    Smoking, heavy alcohol use, and large amounts of coffee are linked with higher homocysteine. Balanced nutrition and regular activity may help.

  • Genetics and special populations

    Variants in enzymes such as MTHFR or CBS and classical homocystinuria can cause marked increases. Pregnancy and older age can also influence results.

2026

References

  1. McGill University Health Centre. (2015, May 04). Homocysteine Total (Task CD 699180). Laboratory reference ranges.
  2. Arnett, D. K., Blumenthal, R. S., Albert, M. A., Buroker, A. B., Goldberger, Z. D., Hahn, E. J., ... Ziaeian, B. (2019). 2019 ACC/AHA guideline on the primary prevention of cardiovascular disease. Journal of the American College of Cardiology, 74(10), e177–e232.
  3. Morris, A. A. M., Kožich, V., Santra, S., Andria, G., Ben-Omran, T., Chakrapani, A., et al. (2017). Guidelines for the diagnosis and management of cystathionine beta-synthase deficiency. Journal of Inherited Metabolic Disease, 40(1), 49–74.
  4. Cuker, A., De Sanctis, Y., Crowther, M., Garcia, D. A., Kahn, S. R., Kearon, C., ... American Society of Hematology. (2020). American Society of Hematology 2020 guidelines for management of venous thromboembolism: Thrombophilia testing. Blood Advances, 4(22), 5833–5858.