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Platelet Aggregation with ADP, Luminescence

Complete Blood Count

ADP-induced platelet aggregation, luminescenceADP lumi aggLumi-aggregometry, ADP

Review status

Currently under review

Pending specialist review and validation.

What it shows

This test evaluates how well your platelets activate and release stored chemicals when stimulated by ADP, a natural signal that triggers platelets to clump. A luminescence method is used to detect ATP released from platelet granules, which reflects the secretion response that accompanies aggregation.

It is performed on a blood sample as part of a comprehensive platelet function study. The result helps your care team see whether your platelets respond appropriately through the ADP pathway and whether the secretion step of platelet activation is intact.

Why it matters

Doctors use this test when there are symptoms such as easy bruising, nosebleeds, heavy menstrual bleeding, or unexpected bleeding with procedures. It helps evaluate for inherited or acquired platelet function disorders and can contribute to assessing the effect of medicines that block the ADP pathway.

Results are interpreted together with other platelet tests, your blood count, and your history. Understanding your platelet response can guide decisions about holding medications before surgery, choosing treatments for bleeding, and deciding if more specialized testing is needed.

Understanding your results

Your result reflects how strongly your platelets responded to ADP and how much ATP they released. A weaker than expected response can be due to antiplatelet medications, recent use of certain over the counter drugs or supplements, low platelet granule content, or inherited changes in the ADP receptor. A robust response may be normal for you, but interpretation depends on the pattern seen with other agonists and your clinical situation.

If your result is outside the expected range, your clinician may review your medications and supplements, consider repeat testing after a washout period, or add complementary tests such as other aggregation studies, von Willebrand factor testing, or genetic evaluation. Abnormal results do not automatically mean you have a bleeding disorder, and many factors that affect platelets are temporary or reversible.

Reference ranges

0.31.7 nm
All sexes
0 days – 150 years

Reference intervals vary by laboratory, analyzer, methodology, population, and units. The ranges shown here are for education only. Always interpret your results against the reference interval printed on your own lab report.

Factors that could impact Platelet Aggregation with ADP, Luminescence

  • Antiplatelet and other medications

    Drugs that affect platelets can blunt the ADP response. These include P2Y12 inhibitors such as clopidogrel, prasugrel, and ticagrelor, as well as aspirin, many NSAIDs, SSRIs and SNRIs, and valproate. Always tell your care team what you take.

  • Supplements, diet, and substances

    Fish oil and omega 3 products, garlic, ginkgo, turmeric, and alcohol may reduce platelet reactivity. High caffeine intake and certain herbal blends can also influence results. Follow any pretest instructions and disclose supplements.

  • Sample collection and handling

    Platelet function is time sensitive. Prolonged tourniquet time, difficult draws, vibration, temperature extremes, or delays to testing can alter platelet responses. Arrive on time and avoid strenuous exercise just before your draw.

  • Platelet count and blood composition

    Very low platelet counts or marked anemia can affect aggregation measurements. Your clinician will interpret this test alongside your complete blood count and other laboratory findings.

  • Illness, inflammation, and stress

    Acute infection, fever, recent surgery, inflammation, and high physiologic stress can change platelet behavior temporarily. If you are acutely unwell, your clinician may defer or repeat testing.

  • Pregnancy and hormonal influences

    Physiologic changes in pregnancy and with some hormonal therapies can modify platelet responses. Let your care team know if you are pregnant or using hormonal medications.

2026

References

  1. McGill University Health Centre. (2017, June 08). Platelet Agg ADP 10uM/ Luminescence (Task CD 1090586). Laboratory reference ranges.
  2. Clinical and Laboratory Standards Institute. (2008). Platelet function testing by aggregometry; Approved guideline (H58-A). Wayne, PA: CLSI.
  3. Cattaneo, M. (2013). Platelet function testing by light transmission aggregometry: strengths, limitations, and practical tips. Journal of Thrombosis and Haemostasis, 11(6), 1186–1197.
  4. Gresele, P., Harrison, P., Mezzano, D., et al. (2011). Laboratory investigation of platelet function disorders. Haemophilia, 17(S1), 53–62.